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KMID : 0861020180330020079
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Korea Journal of Herbology
2018 Volume.33 No. 2 p.79 ~ p.84
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Immune regulation effects of Gentianae Radix extract in LPS-induced acute inflammatory mice
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Lee Hyo-Jung
Seung Yoon-Cheol
Lee Myung-Sun
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Abstract
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Objective : The immune enhance is the main focus of current society that to increase resistance to invasion by pathogenic species of bacteria in body, stimulate the immune system and possibly protect against cancer or inflammatory disease. The present study aimed to evaluate the effect of Gentianae Radix extract on immune regulation in a LPS-induced mice model of acute inflammation.
Methods : Gentianae Radix extract was administered orally at doses of 200 ㎎/㎏/day or 400 ㎎/㎏/day for 2 weeks before a intraperitoneally injection of LPS (1 ㎎/㎏ of 0.9% saline). After LPS-intraperitoneal injection 3 hours, blood was collected by cardiac puncture under ether anaesthesia from all animals, for the immune regulate efficacy verification based on blood or serum biomarkers (i.e., immune cells, cytokine, PGE2, ROS, and LTB4) analysis.
Results : Compared to the control mice, the Gentianae Radix extract treatments significantly increased the count of immune cells (i.e., wite blood cell, neutrophils, and monocyte), and significantly reduced the lymphocyte. In addition, the Gentianae Radix extract treatments significantly decreased the pro-inflammatory cytokine (i.e., IL-1β, IL-6, and TNF-α), and significantly increased IL-10 of anti-inflammatory cytokine. Furthermore, the Gentianae Radix extracts treatments significantly increased the levels of PGE2 and significantly decreased the levels of ROS, and LTB4.
Conclusions : The results indicate that Gentianae Radix extract alleviated acute inflammatory reaction though regulation of immune meditor. Thus, Gentianae Radix extract may raw material of development a health food and medicine option for the immune enhance.
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KEYWORD
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acute inflammation, cytokine, immune, immune cell, LPS-induced
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